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A Multi-Method and Structure-Based <i>In Silico</i> Vaccine Designing Against <i>Helicobacter pylori</i> Employing Immuno-Informatics Approach

[ Vol. 18 , Issue. 2 ]

Author(s):

Anam Naz*, Tahreem Zaheer, Hamza Arshad Dar, Faryal Mehwish Awan, Ayesha Obaid, Shifa Tariq Ashraf, Rehan Zafar Paracha, Arif Malik and Amjad Ali*   Pages 237 - 247 ( 11 )

Abstract:


<p>Background: <i>Helicobacter pylori</i> infection and its treatment still remain a challenge for human health worldwide. A variety of antibiotics and combination therapies are currently used to treat <i>H. pylori</i> induced ulcers and carcinoma; however, no effective treatment is available to eliminate the pathogen from the body. Additionally, antibiotic resistance is also one of the main reasons for prolonged and persistent infection. </P><P> Aims: Until new drugs are available for this infection, vaccinology seems the only alternative opportunity to exploit against <i>H. pylori</i> induced diseases. </P><P> Methods: Multiple epitopes prioritized in our previous study have been tested for their possible antigenic combinations, resulting in 169-mer and 183-mer peptide vaccines containing the amino acid sequences of 3 and 4 epitopes respectively, along with adjuvant (Cholera Toxin Subunit B adjuvant at 5’ end) and linkers (GPGPG and EAAAK). </P><P> Results: Poly-epitope proteins proposed as potential vaccine candidates against <i>H. pylori</i> include SabA-HP0289-Omp16-VacA (SHOV), VacA-Omp16-HP0289-FecA (VOHF), VacA-Omp16-HP0289- SabA (VOHS), VacA-Omp16-HP0289-BabA (VOHB), VacA-Omp16-HP0289-SabA-FecA (VOHSF), VacA-Omp16-HP0289-SabA-BabA (VOHSB) and VacA-Omp16-HP0289-BabA-SabA (VOHBS). Structures of these poly-epitope peptide vaccines have been modeled and checked for their affinity with HLA alleles and receptors. These proposed poly-epitope vaccine candidates bind efficiently with A2, A3, B7 and DR1 superfamilies of HLA alleles. They can also form stable and significant interactions with Toll-like receptor 2 and Toll-like receptor 4. </P><P> Conclusion: Results suggest that these multi-epitopic vaccines can elicit a significant immune response against <i>H</i>. pylori and can be tested further for efficient vaccine development.</p>

Keywords:

<i>H. pylori</i>, multi-epitope vaccine, poly-epitope peptide vaccine, HLA allele binding, TLR2 receptor, TLR4 receptor.

Affiliation:

Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), Islamabad, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), Islamabad, Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), Islamabad, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), Islamabad, Research Center for Modeling & Simulation (RCMS), National University of Sciences & Technology (NUST), Islamabad, 44000, Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), Islamabad

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